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Pleodorina starrii

Page history last edited by PBworks 14 years ago

 

Images

 

 

Taxonomic characters

clearly distinguished from P. californica, P. japonica and P. indica by its individual cellular sheaths that are spherical in shape and separated from one another or weakly attached to each other and absence of a gradual increase in cell size from anterior to posterior pole of colony (Nozaki et al 2006)

 

 

Morphology / Structure

colonies ovoid or spherical in shape; 32-64 cells embedded at the periphery of the gelatinous matrix; 8-12 or 18-20 obligative somatic cells (at the anterior pole) in 32- or 64-celled colonies, respectively; no gradual increase in cell size

 

 

 

Life history

heterothallic; anisogamous

To induce sexual reproduction (Nozaki et al 2006): actively growing cultures of male and female strains in 12mL VTAC medium were concentrated to 0.2–0.4mL by centrifugation. These two concentrated cultures were mixed with 0.5–0.8mL of Pleodorina mating medium (Nozaki et al. 1989) in a watch glass (60mm in diameter) within Petri dishes (90mmx20mm). Mating reactions occurr 1 or 2 days after mixing male and female colonies in mating medium. Each reproductive cell in a male colony divides successively to form a bundle of male gametes (sperm packet). Sexually mature female colonies are essentially the same as that of the egetative colony. Sperm packets are released from the male colony, swim to the female colony and dissociat into the individual male gametes for syngamy. After 1 day, zygotes secrete a smooth cell wall and turn reddish brown in color after about a week. Mature zygotes are spherical in shape, with a smooth cell wall, and measure 19–28 mm in diameter.

 

Ecology

isolated from Lake Sagami, Japan; temperature: 20-21.5C; ph: 7.7-8.2 (Nozaki et al 2006)

 

 

Availability in culture collections or research labs

 

MCC-NIES

 

 

Media and growth conditions in culture

Nozaki et al 2006: AF-6 medium (Kato 1982) modified by the elimination of CaCO3 and by the addition of 400mg/L MES (Kasai et al. 2004) or VTAC medium (Nozaki et al. 1989, Kasai et al. 2004), kept at 20–22C, under a photon irradiance of 150–200 umol photons/m2/s and with a 14:10 light:dark (LD) or 16:8 LD cycle provided by cool-white fluorescent lamps

 

 

 

Sequence Information

 

Links to other databases

 

Index Nominum Algarum

 

 

 

 

Miscellaneous

 

 

 

 

Selected References

Nozaki H, Ott FD, Coleman AW. 2006. Morphology, molecular phylogeny and taxonomy if two new species of Pleodorina (Volvoceae, Chlorophyceae). J. Phycol. 42: 1072-1080.

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Contributors

Nedelcu AManedelcu@unb.ca

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